Other Benefits of Cannabis

 

Cell death in breast cancer cells


Mol Cancer Ther. 2011 Jul;10(7):1161-72. doi: 10.1158/1535-7163.MCT-10-1100. Epub 2011 May 12.
Cannabidiol induces programmed cell death in breast cancer cells by coordinating the cross-talk between apoptosis and autophagy.
Shrivastava A, Kuzontkoski PM, Groopman JE, Prasad A.
Author information
Abstract

Cannabidiol (CBD), a major nonpsychoactive constituent of cannabis, is considered an antineoplastic agent on the basis of its in vitro and in vivo activity against tumor cells. However, the exact molecular mechanism through which CBD mediates this activity is yet to be elucidated. Here, we have shown CBD-induced cell death of breast cancer cells, independent of cannabinoid and vallinoid receptor activation. Electron microscopy revealed morphologies consistent with the coexistence of autophagy and apoptosis. Western blot analysis confirmed these findings. We showed that CBD induces endoplasmic reticulum stress and, subsequently, inhibits AKT and mTOR signaling as shown by decreased levels of phosphorylated mTOR and 4EBP1, and cyclin D1. Analyzing further the cross-talk between the autophagic and apoptotic signaling pathways, we found that beclin1 plays a central role in the induction of CBD-mediated apoptosis in MDA-MB-231 breast cancer cells. Although CBD enhances the interaction between beclin1 and Vps34, it inhibits the association between beclin1 and Bcl-2. In addition, we showed that CBD reduces mitochondrial membrane potential, triggers the translocation of BID to the mitochondria, the release of cytochrome c to the cytosol, and, ultimately, the activation of the intrinsic apoptotic pathway in breast cancer cells. CBD increased the generation of reactive oxygen species (ROS), and ROS inhibition blocked the induction of apoptosis and autophagy. Our study revealed an intricate interplay between apoptosis and autophagy in CBD-treated breast cancer cells and highlighted the value of continued investigation into the potential use of CBD as an antineoplastic agent.American Association for Cancer Research.

PMID:
21566064
[PubMed - indexed for MEDLINE]

Free full text

Frequently Overlooked Benefits of Cannabis

The basic premise of the over reaching benefits of cannabis is that the vast majority of consumers use cannabis as a wellness aid, to enhance the things that are most important in their lives-- not as an intoxicant, to escape from life. These wellness uses certainly include the treatment of serious illnesses like cancer and epilepsy, and the chronic diseases of modern life like insomnia and anxiety and depression, and preventing Alzheimer's and alleviating chronic pain, but this precious plant provides many other less obvious wellness benefits—like extending patience, promoting gentleness and forgiveness, inspiring creativity, improving the appetite and enjoyment of food,  and countless other ways of making us both healthier and happier. These too are all legitimate wellness uses for cannabis.

Please explore some of the articles we have found and posted for you, which are listed below.